ABSTRACT
The presence of bacteria on the hands of fourty (40) school pupils from two different schools (Amai Primary and secondary School) in Amai, Delta State, was analysed. The reason was to enumerate bacteria isolated from hands of students. Swab samples were collected from hands of pupils in both schools of 20 students each of different sex groups. Microbiological methods was used for the isolation, enumeration and antibiotic test of the isolates. The results showed various isolates of Staphylococcus sp 56 (25.7%), Shigella sp 24 (9.7%), Staphylococcus epidermidis 32 (12.1%), Escherichia coli 41 (15.6%), and Enterococcus sp 36 (11.0%). Staphylococcus aureus 56 (25.3%) and Escherichia coli 41 (15.6%) were the most frequent isolates. The isolation of Shigella sp 24 (9.7%) and Enterococcus sp 36 (11.0%) is of great importance as the isolation of these organisms showed improper faecal wastes disposal around the school environment and also lack of proper clean up after using the convience by the pupils. Testing these isolates to few antibiotics, the isolates were susceptible to Pefloxacin, Gentamycin, Erythromycin, Chloramphenicol, Streptomycin, while resistant to Augmentin, Amoxicillin, and Ampiclox. This study revealed that the students hands were infested with pathogens due negligence of maintenance culture. Those in charge of schools like the principal are advised to keep soap and water for hand washing, while parents on their part should make available hand washing facilities for their children at home since it will add economic value to the society, why Government should enact laws that will make provision of washing hand amenities in all areas compulsory for individuals.